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Journal: Open Research Europe
Article Title: Cell motility influences microfluidics capturing in scRNA-seq
doi: 10.12688/openreseurope.19781.2
Figure Lengend Snippet: Parasites were isolated from the blood of three mice six days post-infection and purified through chromatography. Half of the parasite suspension underwent fast cooling to 0 degrees, using dry ice and ethanol (QUENCHED), while the other half remained at room temperature (RT). Both sets of samples underwent encapsulation using the Chromium controller, followed by library preparation and sequencing as recommended by 10x Genomics.
Article Snippet: In this study we confirmed that encapsulating parasites at room temperature using the
Techniques: Isolation, Infection, Purification, Chromatography, Suspension, Encapsulation, Sequencing